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Held for 12h, dried and calcined at 450 ºC for 4h, the modified cobalt nitrate has been decomposed into cobalt oxide, providing a series of γ-Al2O3 with different Co content (denoted by γ-Al2O3). Preparation of Cu-Zn/γ-Al2O3 (Co) catalyst: the theoretical amount of Co(NO3)2·6H2O and Zn(NO3)2·6H2O are dissolved in water to give a series of Cu and Zn solutions, in which the loaded γ-Al2O3 were immersed. After mixing for 4 h, the mixtures were evaporated, dried and calcined at 250 ºC for 5h to get Cu-Zn/γ-Al2O3 (Co) catalyst.

The resulting solution was stirred at room temperature for 12h, evaporated at 80 °C and 100 °C for removing the water, and then was calcined at 800°C for 4h to give WOx-ZrO2. (2) Analysis of catalyst From the XRD patterns, NiO was highly dispersed on the surface of the carrier when the loading of Ni is in the range of 5-15%; while the surface of the carrier on Ni began to gather to microcrystalline when the loading of Ni is up to 20%. From the experiment, an interesting conclusion can also be given.

Of the nutrients, either NH4+-N or NO3–-N may significantly regulate N2-fixation [7,8]. This conclusion was demonstrated by the RDA analysis in the present study that the FNR had a high negative correlation with NH4+-N or NO3–-N (Table 2). Furthermore, FNR correlated with available K (Table 2), but not with available P, probably due to the stimulation of FNR by K [9]. 2). This indicated that the NFB community structure was influenced by fertilization. 2). Similarly, Zou et al. (2011) found that above soil properties other than TC had significant impact on nitrogen-fixing microflora in a chestnut soil [10].

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